Have been suspended within the emulsion composed of oil, Arabic gum and water within the following proportion 2:1:1.five. The administration was performed by stomach tube. Selenium compounds had been offered to rats at a dose of 5 9 104 mg of selenium/g of b.w. after every day for any period of ten days. Body weights of animals had been measured just about every day before selenium administration and the suitable amount of selenium compound was calculated for every single animal. The administered dose was reasonably higher but it was applied together with the aim of studying toxicity from the new organoselenium compounds. It is actually hard to foresee the toxic dose of a brand new compound as the bioavailability is dependent upon its structure. The dose was selected taking into consideration these applied by other authors which had been integrated in the range from 1 9 104 mg/g b.w.Mal-amido-PEG8-NHS ester Chemscene (Medeiros et al. 2012) to 19.7 9 104 mg/g b.w. (Selamoglu Talas et al. 2008), mainly two 9 104 9 104 mg/g b.w. (ElDemerdash 2004; Agarval and Behari 2007; Naziroglu et al. 2008; Akil et al. 2011a). Rats had free of charge access to typical feed containing sufficient level of selenium and drinking water. Just after the end with the experiment animals have been sacrificed under pentothal narcosis. The study was performed in accordance with statutory bioethical standards and authorized by I Nearby Ethical Commission of Health-related University of Lublin, acceptance no.152754-55-7 Order 65/AM/2004. Preparing of brain samples and measurement of biochemical parameters The samples of brain tissue had been collected. Ten per cent (w/v) tissue homogenates had been ready in 0.1 mol/dm3 Tris Cl buffer, pH = 7.4. Supernatants were obtainedby centrifugation at 5,0009g for 30 min. The ready material was stored at temperature 18 . The following substances have been determined in brain homogenates: total antioxidant status (TAS), activity of antioxidant enzymessuperoxide dismutase (SOD) and glutathione peroxidase (GPx), concentrations of nonenzymatic antioxidantsascorbic acid (AA) and reduced glutathione (GSH) too as concentration of lipid peroxidation markermalonyl dialdehyde (MDA). TAS was measured utilizing diagnostic kit developed by RANDOX and expressed in mmol/g of protein. SOD and GPx activities had been determined applying diagnostic kits RANSOD and RANSEL made by RANDOX and expressed in U/mg of protein and U/g of protein, respectively. GSH concentration was determined using BIOXYTECHGSH400TM kit made by OxisResearchTM and expressed in lg of GSH/mg of protein. AA concentration was determined working with modified Kyaw strategy (Rutkowski and Grzegorczyk 1998) and expressed in lmol of AA/g of protein. MDA concentration was determined making use of Ledwo_ yw et al. z (1986) system and expressed in nmol of MDA/mg of protein. Protein was measured applying technique of Bradford (1976).PMID:35991869 The assays have been performed with use of spectrophotometer SPECORD M40 (Zeiss Jena). Statistical evaluation Statistical analysis was performed employing ANOVA test. Comparisons in between control and seleniumsupplemented groups as well as between seleniumsupplemented groups had been produced working with the Tukey’s HSD test or Dunnett’s T3 test. Values were regarded important with p \ 0.05. The option of multiple comparisons test was dependent on evaluation of variance homogeneity in compared groups which was performed making use of Leven’s test. In the occasion of homogeneous variances Tukey’s system of honestly significant differencesHSD test was applied, whereas when variances in groups were heterogeneous (p \ 0.05 in Leven’s test) T3 Dunnet’s test (applicable inside the case of heterogenous variances) was.